Considerations for Quantitative PCR Assay to Support Biodistribution Study
Time: 2:15 pm
day: Day One
- Tissue sample processing and nucleic acid extraction conditions may have significant impact on DNA recovery, RNA quality and PCR assay data.
- Assess the pros and cons of PCR platforms for qPCR assay: real-time qPCR, droplet dPCR and nanoplate dPCR.
- Evaluate general approaches and considerations to develop qPCR and dPCR massay in regulated environment to support biodistribution study